GENIO/frame
Frameshift Analysis and Sequencing Error Detection
GENIO/frame detects frameshifts in human coding sequences with high accuracy.  The frameshift analysis is based on G+C content dependent interleaved 6-tupel (hexamer) entropy of coding/non coding sequences. The system is extensible to other organism specific word entropies. Our results have shown that GENIO/frame reports single and dual frameshifts with good accuracy below  +/- 10 nucleotides (in most cases) and high specificity. Sensitivity/specificity is nearly independent from G+C content. The minimum sequence length for reliable frameshift detection should be about 150nt.  GENIO/frame results have been evaluated with non-redundant coding sequences of 301 human genes. The results are shown in GENIO/frame GeneTest. I'm grateful for your comments and suggestions.
Step 1: Input nucleotide sequence in FASTA file format
You can input your sequences by cut-and-pasting into the sequence window or (easier) enter a filename to upload.
Input sequences are in FASTA format.  Don't forget the ">" in the first line of your sequence.
NEW: You can input multiple sequences (thousands if needed) separated by greater sign ">" and followed by a
unique sequence name (identifier, number) and a new line.

Sequence window (cut-and-paste). DNA sequence, one-letter code, upper/lower case, spaces/invalid letter ignored:

Sequence file (use Netscape Version >= 2.0):

or load demonstration file GenBank HSNCAMX1, Acc. Z29373 with deletion at position 1951.
(H.sapiens gene for neural cell adhesion molecule L1).

 Choose strand:
 strand    forward 
  reverse 

Step 2: Submit data 

 
Step 3: View and download results 

A new window pops up showing the results.


Problems, suggestions, remarks? Send email to
Niels Mache (mache at struktur dot de)  My home page is here.